Sakura Tissue-Tek Film 4740 User Manual

Revised 08/08/2013 3.1
Section 3
OPERATING INSTRUCTIONS
The Tissue-Tek
®
Film
®
Coverslipper is capable of
performing continuous, rapid coverslipping by simple
operating steps.
Loading of Slides
Slides must be correctly inserted into the baskets in
order to be properly coverslipped. Slides must be loaded
into the baskets, taking the following precautions:
The specimen side of the slide must face forward (toward
the words “UP SIDE” on the basket)
The label end of the slide must be to the outside (top) of
the basket
Each slide must be in parallel grooves
Each basket holds 20 slides; the empty grooves in a
partially filled basket may be anywhere in the basket.
To prevent damage to the specimens, keep the slides
totally immersed in clean xylene when positioned in
the loading station of the instrument.
Preparation of Film
CAUTION: Before using a roll of Coverslipping Film, it
is imperative that the film be allowed to acclimate for
at least one full day (24 hours) to the laboratory
conditions in which it will be used.
The film roll should be removed from the box and the
plastic bag opened fully to allow complete exchange of air
to the film. Alternatively, the film can be removed from the
bag and stored in a cupboard or in the instrument, as long
as it is protected from dust, moisture, and chemical
vapors. Failure to allow complete acclimation may result in
bubbles, curling edges, or poor adherence of the film to
the slide, the degree of which depends upon the specific
conditions (temperature and humidity) in the laboratory in
comparison to the conditions of the film at the time of use.
The end of a new roll of film will be secured with a
piece of adhesive tape. Carefully remove the tape,
ensuring that all adhesive is removed with the tape.
Discard the first 75 cm (2 1/2 feet) of Film before installing
a new roll on the Coverslipper. This will ensure removal of
the blemished area originally secured with tape.
Storage of Unused Film
Avoid storing Film in humid areas. Storage conditions
for Film prior to use:
Relative Humidity: 30 to 70%
Temperature: 10º C to 30º C (50º F to 86º F)
Packaged rolls of film should not be stored near
laboratory chemicals. The solvent fumes will penetrate
through the cardboard box and plastic bag, resulting in
degradation of the Film coatings.
NOTE: We can only recommend the use of Tissue-Tek
Coverslipping Film, Product Code # 4770.
Routine Operation
1. Turn the instrument on by firmly pressing the power
switch to the “I” position. The power switch is located
on the front of the instrument on the control panel.
An audible beep will sound briefly, and the following
message will appear on the LCD screen:
2. Load film if not already done (see Section 2
Installation for film installation procedure).
3. Press the [FILM] key and feed the film so the film tip
comes 1-2 mm past the cutter blade edge.
NOTE: Film will be continuously fed as long as the [FILM]
key is pressed.
a. Depress the front end of the blade cutter handle
with a finger and cut the film.
NOTE: The cutter blade handle must be pressed down
when the “Press [FILM] to feed and cut film” message is
displayed.
b. Remove the film end with forceps or fingers.
c. Close the film door.
4. Load the 12-basket unloading unit located under the
cover on the top of the instrument (see 12-basket
unloading unit installation)
Solvent Setup
Film Setup
Instrument Check
[START] to start

Sakura Tissue-Tek Film 4740 Specifications

General

Brand	Sakura
Model	Tissue-Tek Film 4740
Category	Medical Equipment
Language	English