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Tissue-Tek Polar DM
Sakura Tissue-Tek Polar DM User Manual
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Operat
in
g
Manua
l
Frozen Ti
ssue Sect
ion Pre
paration Ins
trum
ent
Cryostat Microtom
e
2
Table of Contents
Default Chapter
3
Table of Contents
3
Safety Precautions
8
Chapter 1 Basic Knowledge of Instrument
15
Basic Knowledge
16
Explanation of System
16
Installation Method
17
Environmental Factors
17
Name of each Part (Exterior)
18
Name of each Part (Left/Right Panels, Display Area)
20
Name of each Part (Chamber)
21
Handwheel
22
Overview of Handwheel
22
Coordination of Handwheel and Specimen Head
22
Handwheel Lock Tab
23
Unlocking the Handwheel
23
Locking the Handwheel
23
Foot Switch (DM Only)
24
Overview of Foot Switch
24
Connecting the Foot Switch
24
How to Use/Lock the Foot Switch
25
Unlocking the Foot Switch
25
How to Operate the Foot Switch
25
Adjusting the Sectioning Speed
25
Locking the Foot Switch
25
How to Operate the Right Panel (DM Only)
26
How to Operate the Right Panel for Motorized Sectioning
26
Starting Motorized Sectioning
26
Pausing Motorized Sectioning
26
Stopping Motorized Sectioning
26
Adjusting the Sectioning Speed
26
Types of Cryobars and Object Holders
27
Types of Cryobar Removable Plates
27
Setting
27
Types of Object Holders
27
Safety Functions
28
Emergency Stop Switch
28
Actuating the Emergency Stop
28
Resetting the Emergency Stop
28
Blade Guard
29
Recovery Processing
30
Specifications
31
Standard Accessories/Options
32
Explanation of Screens
34
Explanation of Screen Layout
34
Explanation of Top of Screen
34
Explanation of Tab Area
34
Explanation of Tab Screen Area
34
Explanation of Right-Of-Screen Button Area
35
Explanation of Status Display Area
35
Section Tab Screen
36
FREE Mode Screen
36
Temperature Setting 1 Mode/Temperature Setting 2 Mode Screen
37
Motorized Sectioning Tab Screen (DM Only)
38
TRIM Mode Screen
38
FINE Mode Screen
39
PAUSE Mode Screen
40
Tuning Tab Screen
41
Utilities Tab Screen
42
Other Items
43
History Tab Screen
44
Various Settings
45
Setting the Section Thickness
45
How to Change in the Manual Sectioning Mode
45
How to Change in the Motorized Sectioning Mode
45
Setting the TRIM Thickness
46
Registering/Editing in the Temperature Setting FREE Mode
47
How to Set the Chamber Temperature
47
How to Set the Specimen Head Temperature
47
Registering/Editing in the Temperature Setting 1 / Temperature Setting 2 Mode
48
How to Set the Temperature
48
Setting the Home Position Offset
49
Setting the Sectioning End Position
50
Setting the Vacuum Suction Force
51
Setting a Desired Suction Force
51
The Suction Force Is Fixed to 100
51
Setting the Date/Time
52
Setting the Date
53
Setting the Time
53
Setting a Schedule
54
Explanation of Always-On Operation Setting Screen
54
Setting the Cleaning Operation (Defrost Cycle/Ozone Cycle) Start Time
55
Explanation of Scheduled Operation Setting Screen
56
Setting the Operation Ready/Shutdown Times
57
Setting Days off
58
Deleting Days off Setting
58
Setting an Operating Day
59
Deleting an Operating Day Setting
59
Using External Save
60
Backing up to the Instrument
60
Restoring from the Backup in the Instrument
60
Outputting (Exporting) to a USB Memory Device
60
Inputting (Importing) from a USB Memory Device
60
Selecting the Language
61
Displaying the Maintenance Information
61
Setting the Cooling Method
61
Setting Sounds
62
Changing the Sound Volume
62
Changing the Sound
62
Setting Accounts
63
Switching between the Manager List and User List
63
Setting/Changing a Password
63
Various Permission Table
64
Using External Save
65
Backing up to the Instrument
65
Restoring from the Backup in the Instrument
65
Outputting (Exporting) to a USB Memory Device
65
Inputting (Importing) from a USB Memory Device
65
Viewing and Outputting the History
66
Checking and Outputting the Error Log
66
Checking and Outputting the Event Log
66
Software Version
67
Checking the Version
67
Updating
67
Chapter 2 Operating Method
69
Basic Operation Flow
70
Logging on to the System
70
Performing Pre-Operation Checks
71
Turning on the Power
71
Logging on to the Instrument
72
Logging off from the Instrument
72
Returning from the Sleep Mode
72
Returning from the Standby Mode
72
Preparing for Sectioning
73
Checking the Temperature Settings
73
Checking the "FREE" Temperature Settings
73
Changing the Chamber Temperature
73
Changing the Specimen Head Temperature
73
Preparing for Frozen Block Preparation
74
Freezing in the Chamber
74
Setting the Frozen Block on the Specimen Head
75
Adjusting the Specimen Head
76
Adjusting the Specimen Head Orientation (Angle)
76
Changing the Disposable Blade
77
Using the High Profile Type
77
Adjusting the Lateral Movement of the Blade Holder
78
Using the Low Profile Type
79
How to Set the Low Profile Adapter
79
Sectioning
80
Preparing for Manual Sectioning (Final Shaving)
80
Press the Home Position Key to Move the Specimen Head to the Sectioning Start Position
80
Cutting the Specimen to Flat Surface (Rough Shaving)
80
Checking the Section Thickness for Sectioning (Final Shaving)
80
Manually Sectioning the Specimen (Final Shaving)
81
Sectioning Manually
81
Sectioning with the Anti-Roll Rake
82
Sectioning with the Anti-Roll Plate
83
After Processing
84
Clean the Interior of the Chamber Using the Supplied Nozzle
84
Performing Cleaning Operation
85
Cancelling Cleaning Operation
86
Defrosting Manually
87
Cancelling Manual Defrost Cycle
87
Performing Ozone Cycle Manually
88
Cancelling Manual Ozone Cycle
88
Shutting down the Instrument
89
Shutting down the Instrument Manually
89
Manual Shutdown When "Always-On Operation" Is Selected
89
Manual Shutdown under "Scheduled Operation
90
Cancelling Manual Shutdown
91
Shutting down the Instrument Automatically
92
Cancelling Automatic Shutdown
93
Other Operations
94
When the Instrument Detects an Abnormality During the Ozone Disinfection Cycle
94
Errors Notified after Completion of Ozone Decomposition
94
Errors Notified When Ozone Might Still be Inside the Chamber
94
Preparing for Motorized Sectioning (DM Only)
95
Preparing for Sectioning
95
Selecting an Motorized Sectioning Mode
95
Moving the Specimen Head to the Sectioning Start Position
96
Cutting the Specimen to Flat Surface (Rough Shaving) in the TRIM Mode
96
Checking the Section Thickness
96
Sectioning Automatically (DM Only)
97
How to Operate the Right Panel
97
How to Operate the Foot Switch
97
Adjusting the Anti-Roll Plate
98
Adjusting the Forward/Backward Position of the Anti-Roll Plate
98
Adjusting the Angle (Lateral Inclination) of the Anti-Roll Plate
98
Adjusting the Blade Holder
99
Adjusting the Blade Angle (Relief Angle)
100
Removing the Blade Holder from the Chamber
101
Setting the Blade Holder in the Chamber (Normal)
101
Setting the Blade Holder in the Chamber (Cryo3 Adapter Is Used)
102
Turning on the Chamber LED Lights
103
Assisting with Vacuum
103
Draft Generator
104
Starting the Draft Generator
104
Stopping the Draft Generator
104
Changing the Suction Force of the Draft Generator
104
Setting the Parking Plate
105
Setting the Heat Extractor
105
Plastic Object Holder
106
Using the Plastic Object Holder
106
Adapter for Cryo3 Object Holder
107
Using Adapter for Cryo3 Object Holder
107
Freeze the Block Using the PINO Hyfluid Method
107
Chapter 3 Maintenance/Inspection
109
Daily Care and Inspection
110
Cleaning the Chamber
110
Cleaning the Chamber Drain Pipe
110
Cleaning the Specimen Head
110
Cleaning the Holder Installation Position
110
Cleaning the Operation Panel and Instrument Exterior
110
Setting the Waste Tank
111
Cleaning the Waste Tank
111
How to Install the Vacuum Filter Set
112
Replacing the Vacuum Filter Set
113
Cleaning the Vacuum Hose
113
How to Set the Draft Generator
114
Draft Generator Error Codes
114
Replacing the Draft Generator Filter
115
How to Disassemble/Set and Clean the Blade Holder
116
Disassembling/Cleaning the Blade Holder
116
Disassembling/Cleaning the Stage
117
Disassembling/Cleaning the Wave Washer and Lever Locking Bracket of the Blade Holder (Base)
117
How to Disassemble/Set and Clean the Anti-Roll Plate
118
How to Remove the Anti-Roll Plate and Vacuum Suction Port
118
How to Remove Only the Anti-Roll Plate
118
How to Install the Anti-Roll Plate
118
Disassembling and Assembling the Anti-Roll Plate
119
Replacing the Glass of the Anti-Roll Plate
119
Cleaning the Vacuum Suction Port
119
Cleaning the Anti-Roll Rake
120
Disassembling/Cleaning the Anti-Roll Rake
120
Cleaning the Object Holder
120
Cleaning the Window Gasket
120
Replacing the Ozone Lamp
120
Cleaning the Air Intake Grille
121
Replacement of the Brine Solution (Auxiliary Cooling Fluid)
121
Chapter 4 Troubleshooting
123
Troubleshooting
123
Before Suspecting a Trouble ~ Instrument Conditions and Remedial Actions
124
When Error/Alarm/Other Message Screens Appear
127
Error Codes Displayed During System Operation
128
Glossary of Terms
130
Explanation of Terms
130
Decontamination Statement
131
Warranty Period and Repair
132
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Sakura Tissue-Tek Polar DM Specifications
General
Brand
Sakura
Model
Tissue-Tek Polar DM
Category
Medical Equipment
Language
English
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