Name: Sakura Tissue-Tek Polar DM
Brand: Sakura
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Introduction

The frozen tissue section preparation

instrument cryostat microtome Tissue-Tek

Polar

(herein

after simply referred to as

"the Polar

instrument") is an instrument

to section frozen tissues.

Carefully read this Operating Manual

and use the Polar

instrument correctly

so that its performance can be utilized

fully.

Default Chapter3
- Table of Contents3
Safety Precautions8
Chapter 1 Basic Knowledge of Instrument15
Basic Knowledge16
- Explanation of System16
Installation Method17
- Environmental Factors17
Name of each Part (Exterior)18
Name of each Part (Left/Right Panels, Display Area)20
Name of each Part (Chamber)21
Handwheel22
- Overview of Handwheel22
  - Coordination of Handwheel and Specimen Head22
- Handwheel Lock Tab23
  - Unlocking the Handwheel23
  - Locking the Handwheel23
Foot Switch (DM Only)24
- Overview of Foot Switch24
- Connecting the Foot Switch24
- How to Use/Lock the Foot Switch25
How to Operate the Right Panel (DM Only)26
- How to Operate the Right Panel for Motorized Sectioning26
Types of Cryobars and Object Holders27
- Types of Cryobar Removable Plates27
  - Setting27
- Types of Object Holders27
Safety Functions28
- Emergency Stop Switch28
  - Actuating the Emergency Stop28
  - Resetting the Emergency Stop28
- Blade Guard29
- Recovery Processing30
Specifications31
Standard Accessories/Options32
Explanation of Screens34
- Explanation of Screen Layout34
Section Tab Screen36
- FREE Mode Screen36
- Temperature Setting 1 Mode/Temperature Setting 2 Mode Screen37
Motorized Sectioning Tab Screen (DM Only)38
- TRIM Mode Screen38
- FINE Mode Screen39
- PAUSE Mode Screen40
Tuning Tab Screen41
Utilities Tab Screen42
- Other Items43
History Tab Screen44
Various Settings45
- Setting the Section Thickness45
  - How to Change in the Manual Sectioning Mode45
  - How to Change in the Motorized Sectioning Mode45
- Setting the TRIM Thickness46
- Registering/Editing in the Temperature Setting FREE Mode47
  - How to Set the Chamber Temperature47
  - How to Set the Specimen Head Temperature47
- Registering/Editing in the Temperature Setting 1 / Temperature Setting 2 Mode48
  - How to Set the Temperature48
- Setting the Home Position Offset49
- Setting the Sectioning End Position50
- Setting the Vacuum Suction Force51
  - Setting a Desired Suction Force51
  - The Suction Force Is Fixed to 10051
- Setting the Date/Time52
  - Setting the Date53
  - Setting the Time53
- Setting a Schedule54
- Selecting the Language61
- Displaying the Maintenance Information61
- Setting the Cooling Method61
- Setting Sounds62
  - Changing the Sound Volume62
  - Changing the Sound62
- Setting Accounts63
- Viewing and Outputting the History66
  - Checking and Outputting the Error Log66
  - Checking and Outputting the Event Log66
- Software Version67
  - Checking the Version67
  - Updating67
Chapter 2 Operating Method69
Basic Operation Flow70
Logging on to the System70
- Performing Pre-Operation Checks71
- Turning on the Power71
- Logging on to the Instrument72
- Logging off from the Instrument72
- Returning from the Sleep Mode72
- Returning from the Standby Mode72
Preparing for Sectioning73
- Checking the Temperature Settings73
- Checking the "FREE" Temperature Settings73
  - Changing the Chamber Temperature73
  - Changing the Specimen Head Temperature73
- Preparing for Frozen Block Preparation74
- Freezing in the Chamber74
- Setting the Frozen Block on the Specimen Head75
- Adjusting the Specimen Head76
  - Adjusting the Specimen Head Orientation (Angle)76
- Changing the Disposable Blade77
Sectioning80
- Preparing for Manual Sectioning (Final Shaving)80
- Manually Sectioning the Specimen (Final Shaving)81
- Sectioning Manually81
- Sectioning with the Anti-Roll Rake82
- Sectioning with the Anti-Roll Plate83
After Processing84
- Clean the Interior of the Chamber Using the Supplied Nozzle84
- Performing Cleaning Operation85
  - Cancelling Cleaning Operation86
- Defrosting Manually87
  - Cancelling Manual Defrost Cycle87
- Performing Ozone Cycle Manually88
  - Cancelling Manual Ozone Cycle88
- Shutting down the Instrument89
- Shutting down the Instrument Manually89
- Shutting down the Instrument Automatically92
  - Cancelling Automatic Shutdown93
Other Operations94
- When the Instrument Detects an Abnormality During the Ozone Disinfection Cycle94
  - Errors Notified after Completion of Ozone Decomposition94
  - Errors Notified When Ozone Might Still be Inside the Chamber94
- Preparing for Motorized Sectioning (DM Only)95
- Sectioning Automatically (DM Only)97
  - How to Operate the Right Panel97
  - How to Operate the Foot Switch97
- Adjusting the Anti-Roll Plate98
  - Adjusting the Forward/Backward Position of the Anti-Roll Plate98
  - Adjusting the Angle (Lateral Inclination) of the Anti-Roll Plate98
- Adjusting the Blade Holder99
- Turning on the Chamber LED Lights103
- Assisting with Vacuum103
- Draft Generator104
- Setting the Parking Plate105
- Setting the Heat Extractor105
- Plastic Object Holder106
  - Using the Plastic Object Holder106
- Adapter for Cryo3 Object Holder107
  - Using Adapter for Cryo3 Object Holder107
- Freeze the Block Using the PINO Hyfluid Method107
Chapter 3 Maintenance/Inspection109
Daily Care and Inspection110
- Cleaning the Chamber110
  - Cleaning the Chamber Drain Pipe110
- Cleaning the Specimen Head110
  - Cleaning the Holder Installation Position110
- Cleaning the Operation Panel and Instrument Exterior110
- Setting the Waste Tank111
- Cleaning the Waste Tank111
- How to Install the Vacuum Filter Set112
- Replacing the Vacuum Filter Set113
  - Cleaning the Vacuum Hose113
- How to Set the Draft Generator114
- Draft Generator Error Codes114
- Replacing the Draft Generator Filter115
- How to Disassemble/Set and Clean the Blade Holder116
- How to Disassemble/Set and Clean the Anti-Roll Plate118
- Cleaning the Anti-Roll Rake120
  - Disassembling/Cleaning the Anti-Roll Rake120
- Cleaning the Object Holder120
- Cleaning the Window Gasket120
- Replacing the Ozone Lamp120
- Cleaning the Air Intake Grille121
- Replacement of the Brine Solution (Auxiliary Cooling Fluid)121
Chapter 4 Troubleshooting123
- Troubleshooting123
Glossary of Terms130
- Explanation of Terms130
Decontamination Statement131
Warranty Period and Repair132

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Sakura Tissue-Tek Polar DM Specifications

General

Brand	Sakura
Model	Tissue-Tek Polar DM
Category	Medical Equipment
Language	English

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