SLIDE HANDLING
4.2 Revised 08/08/2013
2. Place the slide on a flat surface. Gently press the Film
surface with a paper towel for five to ten seconds to
absorb excess xylene and to tease out any bubbles.
(Do not work out a bubble using any sharp object,
which would mar the surface of the Film.)
Method “C: removes and prevents the reuse of that
piece of coverslipping film. This would be used to
completely remove the coverslipping film. This method
would also be utilized if a bubble near the edge or edge
release is observed.
1. Place slide in acetone for 30 seconds to five minutes
to loosen the film.
2. Immediately place slide in 50% xylene/50% acetone
solution for 30 to 45 seconds.
3. Carefully peel the loosened coverslipping film from
the slide surface. If film is difficult to remove, repeat
steps 1 and 2.
4. Immediately place the slide in xylene for one minute
and gently agitate.
5. Transfer the slide to a second xylene rinse for one
minute to ensure complete removal of coverslipping
resin.
6. Re-coverslip the specimen slide using recommended
operating procedures.
Storage of Coverslipped Slides
This section identifies the need to:
1. Store slides front-to-back.
2. Have slides be in direct contact with each other.
3. Avoid separating slides or storing them flat in trays.
4. Avoid humidity greater than 50%.
5. Avoid temperatures greater than 77°F and less than
67°F.
6. Avoid long exposure to fluorescent light or sunlight.
7. Conduct annual, random examination of archived
slides.
It is also noted that the real time stability data goes
back to 1988. As of June 2013, this represents 25 years.
As stated initially, long term negative conditions need
to be addressed at the time of initial coverslipping.
Review staining procedures and address deviations from
Sakura recommendations as immediately as possible.
Technical support is also available at 800-725-8723,
menu option “2”.
As a reference, the CLIA guidelines for archived slides
are as follows:
Cytology Slides
Negative, unsatisfactory: 5 years
Suspicious, positive: 5 years
Histology Slides:
All stained slides: 10 years
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