36 | P a g e
OPERATION (CONTINUED)
PLACE THE BACTRON IN OPERATION
The BACTRON requires an activated catalyst for operation. Catalyst cartridges come from the
factory ready for use. However, if the cartridge has been stored for 6 months or longer, a bake out
of at least 8 hours at 200C will be needed to reactivate the catalyst. The BACTRON requires a
continual supply of AMG to set up and operate. The BACTRON300 needs approximately 200 psi of
AMG for its purge of the workspace chamber atmosphere. The BACTRON600 and BACTRON900
require around 400 psi of AMG for the setup purge of the chamber and side storage incubator.
The BACTRON should run for 24 hours prior to loading samples. This ensures the stability of both
the anaerobic atmosphere and incubator air temperature.
Carry out the following steps and procedures to place the BACTRON in operation:
1. Plug the power cord receptacle into to power inlet on the BACTRON power supply panel.
The panel is located on the back of the BACTRON.
2. Insert the power cord plug into the room power source outlet.
3. Power the BACTRON by placing the main control panel power switch in the On ( I )
position.
a. The power switch and all digital displays should illuminate and remain on.
b. The control panel indicator lights will briefly illuminate.
c. A vacuum pump plugged into the BACTRON will turn on and run briefly to establish a
supply of vacuum in the BACTRON internal supply line. If the vacuum pump runs near
continually at higher altitudes (above 6500 feet / 2000 meters) the BACTRON requires
adjustment. Please contact Sheldon Technical Support.
Note: If you are using a building vacuum system, supply at least -25inHg (-63.5cmHg) of vacuum
to the BACTRON VAC IN port now.
4. Carry out the following procedures in the Operations section to ready the BACTRON for
use.
Establish an Anaerobic Atmosphere page 37
Install the Sleeve Assemblies page 41
Chamber Entry page 41
Verify the Anaerobic Atmosphere page 42
Exiting the Chamber page 46
Set the Incubator Temperature page 47
Set the Over Temperature Limit page 50
Optional: Verify Incubator Temperature Display Accuracy page 48
Load Samples page 36
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