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OPERATION (CONTINUED)
VERIFYING AN ANAEROBIC ATMOSPHERE
This procedure verifies that an anaerobic atmosphere has been established
prior to placing anaerobe samples in the workstation.
1. After entering the workspace chamber, open one of the Oxoid brand
anaerobic monitoring strip packages.
2. If the strip remains white, the chamber atmosphere is free of oxygen
and ready to use.
a. Exit the chamber using the steps described in the Exiting the
Chamber procedure on page 46.
b. The verification procedure is now complete.
- Or -
3. If the strip turns partly or completely pink, exit the chamber using the
Exiting the Chamber procedure steps to avoid introducing more oxygen
into the chamber.
4. The chamber must be further purged.
a. Loosen the right arm port door to create a controlled leak that aerobic atmosphere can
be expelled through. See the Establish an Anaerobic Environment procedure steps
on page 39.
b. Allow an additional 50 psi or 30 minutes worth of AMG to flush through the chamber.
Note: The sleeve assemblies can be left on the BACTRON during this second purge.
5. When 30 minutes has elapsed or 50 psi of AMG have been flushed through the chamber
(whichever comes first), re-enter the chamber using correct entry procedures, and open a
second anaerobic monitoring strip.
a. If the second anaerobic indicator strip shows less pink than before, repeat steps 4a and
4b to finish purging the chamber.
b. If the second strip turns completely pink or shows the same degree of coloring as the
first, contact Sheldon Technical Support for assistance. See page 7. Also, see the
optional troubleshooting procedure on the next page for steps to identify possible
sources of oxygen in the workstation.
Figure 28: Oxygen
Free Indication
(Anaerobic)
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