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OPERATION (CONTINUED)
TEMPERATURE ACCURACY VERIFICATION
Note: Performing a temperature accuracy verification requires a temperature reference device.
Please see the Reference Sensor Device entry on page 12 for the device requirements.
Optional: Each BACTRON incubator was calibrated at the factory at 37ºC. A verification of the
display accuracy may be carried out when preparing the incubator for use or when required by your
laboratory or production protocol. The verification procedure compares the displayed incubator
temperature with the actual chamber air temperature, as provided by a calibrated reference device.
If a difference between the actual and displayed temperatures is discovered, perform a temperature
calibration. Please see the Calibrate Temperature Display procedure on page 70 in the User
Maintenance section.
Temperature display accuracy must be verified separately for each incubator in the BACTRON900.
Probe
A reference device sensing probe may be introduced into an incubator
through the door space. In the cabinet style incubators the wire may be
introduced through the finger hole in either door.
Place the sensor probe head as close as possible to the geometric center of
the incubator. A thermocouple sensor probe sleeve may be taped to the
shelving using non-stick, non-marking tape, as long as the exposed copper
end is 2 inches (5cm) away from the shelf (see Figure 29). An exposed
sensor probe in direct contact with the shelving may experience heat sinking,
which can result in an inaccurate temperature reading.
In a side storage incubator, carefully close the incubator door over the probe
wire, and, if possible, latch the door. Use non-stick, non-marking tape to
secure the wire and to seal any gaps.
Temperature Stability
After introducing and placing the temperature probe, allow the incubator to operate undisturbed for
8 hours (for example, overnight) prior to performing the verification. A 24-hour wait is strongly
recommended if the BACTRON has just been installed in a new location.
The incubator must operate at its verification temperature set point for at least 1 hour with no
fluctuations of ±0.1°C or greater in order to be considered stabilized. Failure to wait for
stabilization will result in an inaccurate verification. If the incubator door is opened during the
verification the chamber must be allowed to re-stabilize before continuing.
Figure 29: Probe End
Taped 2 inches (5cm)
From Shelf Surface
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