53 | P a g e
OPERATION (CONTINUED)
LOAD SAMPLES
Sheldon Manufacturing recommends waiting 24 hours after establishing an anaerobic atmosphere
before loading samples into the unit.
Airtight containers can introduce significant amounts of oxygen into the anaerobic environment of
the BACTRON. Whenever possible, closed containers placed in the pass box should be loose-
capped or ventilated to allow the pass box cycles to draw oxygen from the containers. Syringes
should be loosened and sealed containers partly opened, if permitted by your laboratory or
production protocol.
The pass box sliding shelf can hold and transport up to 252 plates.
Place samples and other media containers on the shelves inside each incubator as evenly spaced
as possible. Good spacing allows for atmosphere circulation and a higher degree of temperature
uniformity. If the anaerobes sensitive to heat are being cultivated, it may be necessary to place an
empty Petri dish or plate at the bottom of each stack.
This concludes the Placing the BACTRON into Operation portion of the Operation Section
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