Shel lab BACTRON300 - Page 38

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OPERATION (CONTINUED)
Procedure continued on next page
Establish an Anaerobic Atmosphere (Continued)
6. Verify the interior and exterior pass box doors are closed and securely
latched.

7. Check that the arm port foot pedal control assembly is connected to
both arm ports and the VAC and GAS out ports on the BACTRON.

8. Make sure the AMG supply cylinder regulator is set to provide 15 to 20
psi of gas flow.
AMG 
9. Open the AMG supply valve all the way to start a flow of gas to the
BACTRON.
a. Nitrogen and other inert gases will not successfully purge
workstation.
10. Open and remove the right arm port door.
11. Unwrap and install a fresh or reactivated catalyst cartridge on the left
wall inside the workspace chamber.
a. The catalyst cartridge will grow warm in the presence of oxygen
and AMG.
Note: You may open the left arm port door to install the catalyst. Close and
seal the arm port door immediately after finishing, as per the Installing the
Arm Port Doors procedure on page 24. Leaving the left arm port unsecured
and unsealed may cause AMG to “stream” through the chamber and exit
straight out to the left door. During the purge, AMG should build up on the left
side of the chamber, gradually pushing aerobic atmosphere out the right arm
port door.