ProStar 430 Autosampler Control Manual - 8 - 03-914947-33:2
NOTE: The 10 000 µL syringe can only be used with the User Program mode of injection
and not with the standard injection program.
Tubing volume (1 to 200 microliters)
Enter the volume of the tubing and needle connected to the
injection valve. The Varian ProStar 430 uses this value for
calculations of flush volumes.
NOTE: With standard loop, syringe and tubing, the injection volumes are:
Full loop: injection volume = loop volume
Partial loopfill: maximum injection volume = 50% of loop volume
µL pick-up: maximum injection volume = 1/2 * (loop volume – 3 x needle tubing
volume)
Refer to the Autosampler Operation Manual to know more about the calculation
of the maximum injection volume according to the loop, syringe and tubing
volumes.
Check Use transport vials to activate the two following features:
First transport vial (1 to 4)
Enter the transport vial number of the vial containing the
transport solvent (mobile phase to avoid disturbance of the
chromatogram with a solvent peak). During µL pick-up
injections, this transport solvent will be used to purge the
sample loop of wash solvent prior to sample loading.
Last transport vial (1 to 4)
Enter the transport vial number of the last transport vial. The
ProStar 430 scans the transport vial area starting with the first
location selected and ending with this location. If no vial is
found, an error will be reported.
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