Waters ACQUITY UPLC Care And Use Manual

1
[ CARE AND USE MANUAL ]
CONTENTS
I. INTRODUCTION
II. GETTING STARTED
a. Column Connectors
b. Column Installation
c. Column Equilibration
d. Procedure for Using New,
Out-of-Box Columns
e. eCord Installation
f. Initial Column Efficiency Determination
g. Column QR Code
III. COLUMN USE
a. Sample Preparation
b. Recommended Mobile Phases
c. Recommended Injector Wash Solvents
d. pH Range
e. Pressure
f. Temperature
g. Mixer Options
h. Flow Rate
IV. COLUMN CLEANING, REGENERATION,
AND STORAGE
a. Cleaning and Regeneration
b. Storage
V. eCORD INTELLIGENT CHIP TECHNOLOGY
a. Introduction
b. Installation
VI. ADDITIONAL INFORMATION
a. Band Spreading Minimization
VII. CAUTIONARY NOTE
I. INTRODUCTION
Thank you for choosing a Waters™ ACQUITY™ UPLC™ and/or
ACQUITY Premier Oligonucleotide BEH C
18
Column designed
specifically for use on ACQUITY UPLC and ACQUITY Premier
Systems. The separation of detritylated oligonucleotides on
Waters second generation of hybrid-silica BEH Technology™
particles are based on the well established method on
ion-pair, reversed-phase chromatography. ACQUITY UPLC
and ACQUITY Premier Oligonucleotide BEH C
18
Columns
are available in several configurations to address different
application needs. The ACQUITY UPLC and ACQUITY Premier
Oligonucleotide BEH C
18
packing material is manufactured in
a cGMP, ISO 9002 certified plant using ultra pure reagents.
Each batch of ACQUITY UPLC and ACQUITY Premier
Oligonucleotide BEH C
18
material is chromatographically tested
with acidic, basic, and neutral analytes and the results are held
to narrow specification ranges to assure excellent, reproducible
performance. In addition, every column is individually tested
and the associated Performance Test Chromatogram and
Certificate of Acceptance information is available through the
attached eCord Intelligent Chip Technology.
Note: Optimum performance of ACQUITY UPLC and ACQUITY
Premier Oligonucleotide BEH C
18
Columns is best assured using
an appropriately configured Waters ACQUITY UPLC Systems
(e.g., See Section II, g). Consequently, use of ACQUITY UPLC
and ACQUITY Premier Oligonucleotide BEH C
18
Columns on
conventional HPLC systems is not recommended.
ACQUITY UPLC and ACQUITY Premier Oligonucleotide BEH C
18
Columns

Waters ACQUITY UPLC Specifications

General

Technology	Ultra Performance Liquid Chromatography (UPLC)
Flow Rate Range	0.001 to 2.000 mL/min
Maximum Pressure	15, 000 psi (1, 034 bar)
Temperature Range	4 °C to 65 °C
Data Sampling Rate	up to 80 Hz
Detector Options	Photodiode Array (PDA), UV/Vis, Fluorescence, Evaporative Light Scattering
Detection Wavelength Range	190 to 800 nm
Column Compatibility	UPLC Columns (1.7 μm particle size)