WPI EVOM Manual - TEER Calculations

2 World Precision Instruments
• Select Filename to enter a new name.
• Press Clear .
• Use the on screen keypad to enter a new name.
• Press Enter .
NOTE: Auto indexing changes the name to a numeric sequence formatted with
the name, a prex and a sequential number (name_prexN).
3. Press Setup to access the Setup menu.
• Select Mode–Set the mode to PD.
• Select Plate–Choose the plate size.
• Display Units– Select the display units in volts or millivolts. (The normal mode
is millivolts.)
• Calibrate (zero) the electrodes in saline. Press the Probe Null button. Push the
Null button. Wait for the second message stating ”Solution value 0.0” before
returning. This action removes any voltage osets on the electrodes.
• Press Return to navigate back to the main menu.
4. Insert a USB thumb drive into the USB port on the side of the EVOM™ Manual
and begin measuring by pressing the foot switch or touching the Store button on
the screen. Tap the Next Well area on the right side of the screen to advance to
the next well. Repeat the sampling process.
5. Once the last well has been recorded, the le storing selection notication
appears. Press Store . A system message will report if the USB thumb drive is
usable, or it will display an error. If the USB thumb drive is not plugged in or it
is not recognized, a warning message will appear. Press Cancel and try another
drive. The Store function cannot be used on an unrecognized drive. EVOM™
Manual should respond with “File saved” within 15 seconds. If “Saving le...” is still
seen after 20 seconds, press the Return key and use another USB drive. Once a
le has been saved, open the le on a computer to verify the contents.
NOTE: The le saving routine can take up to 15 seconds to complete. See the USB
cautions on page 5.
6. Remove the USB thumb drive to transfer the data le to your computer as a CSV
le (Excel).
TEER Calculations
To compute TEER, multiply the measured resistance by the surface area’s listed below.
For example, a 12 mm insert measures 565 Ω, the TEER is 565Ω * 1.13 or 638 Ω.
• 6 well plate (24 mm inserts) 4.52 cm
2
• 12 well plate (12 mm inserts) 1.13 cm
2
• 24 well plate 6.5 mm inserts) 0.3316 cm
2
• 96 well plate (4.3 mm inserts) 0.145 cm
2