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Axiovert 25
Page 67 (Change of Fluorescence Reflector)
Zeiss Axiovert 25 - Change of Fluorescence Reflector; Operating the FI Reflector Module
76 pages
Manual
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Table of Contents
Main Page
Table of Contents
3
List of Figures
6
General View
9
Hints on Instrument Safety
11
Description
13
Designation, Purpose
13
Description of the Unit
13
Mechanical Design
15
Optical Design
16
Technical Data
17
Overview of Components
20
Microscope Configurations
20
Accessories for the Microscope Configurations
25
Function Elements
30
Operation
35
Assembly of the Instrument
35
Unpacking
35
Installation
36
Preparations
36
Screwing in of Objectives
36
Insertion of Ring Diaphragm Sliders
37
Attachment of the HBO 50 Illuminator
37
Attachment of the Specimen Guide M
38
Connection to the Line
39
Inserting the Eyepieces
40
Compensating
40
Using the Binocular Tube
41
Inserting an Eyepiece Reticle
41
Positions of the Binocular Tube
41
Positioning/Switching on the Instrument
42
Start-Up
42
Use of LD Objectives
43
Working with LD Objectives
43
Illumination and Contrasting Techniques
44
Use of Condensers
44
Height Adjustment of the Condenser 0.55
45
Brightfield Illumination
45
Phase Contrast
46
Viewing in Phase Contrast
46
Illustration of Phase-Contrast Adjustment
46
Insertion of the Ring Diaphragm
47
VAREL Contrast
48
VAREL Contrast Observation
48
VAREL Contrast with Microtiter Plates
48
Preparing the Var/H/Var Slider for Phase Contrast
49
Pupil Images in VAREL Contrast
49
Reflected Light Fluorescence
50
Working with the Specimen Guide M and Holding Frames
51
Selection of Available Holding Frames
52
Using the Focusing Eyepiece
53
Selected Eyepiece or Object Measuring Plates
53
Working with Micromanipulators
54
Stage Replacement
55
Glass Stage
56
Heating Stage
56
Insertion of the Glass Stage
56
Documentation
57
Photomicrography
57
Video Microscopy
58
Maintenance
59
Troubleshooting and Service
60
Checking the Line Voltage
60
Change of Halogen Lamp
61
Change of HBO 50 Lamp
62
Safety Notes for Use
63
Change of Lamp
63
Lamp Adjustment
65
Change of Reflectors FI
66
Change of Fluorescence Reflector
67
Operating the FI Reflector Module
67
Service
68
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