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4titude 4s3 - Run Mode Operation; Stand-by Mode Operation

4titude 4s3
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13
4s3™ Semi-Automatic Sheet Heat Sealer
4s3™ Semi-Automatic Sheet Heat Sealer, Operation Manual V1.0
3.4 Operation - "Run" Mode
Switch on the unit. The unit will automatically begin to warm up the heating platen to the last set temperature.
The display shows the temperature increase in real time with the amber LED ashing.
When the heating platen reaches the set temperature an audible beep is emitted and the amber LED remains
permanently illuminated.
The system is ready for sealing.
Press the “OPERATE” (UP) button to open drawer and then the sample plate can be loaded onto the appropriate
plate support adapter, please refer to section 4.2.
Place the sealing lm over the plate, ensuring that the sealing surface is face down.
Note:
When using heat sealing lms that have a tendency to curl, e.g. 4titude
®
Peel Heat Seal (4ti-0521), DMSO
Resistant Peel Heat Seal (4ti-0587) and Clear Weld Heat Seal Mark II (4ti-0575), use the 4s3 Sealing Frame
(4ti-0612) to hold down the seal.
The Sealing Frame is supplied with the 4s3™ Plate Support Adapter, PCR 96 (4ti-0625, optional extra) and
also available as separate item (4ti-0612).
For deep well plates place the 4s3™ Weighted Sealing Platen (4ti-0602, optional extra) on top of the sealing
lm.
For most other plates we recommend the use of the 4s3 Sealing Frame (4ti-0612).
Press the “OPERATE” (UP) button with drawer open, plate and adapter loaded. The drawer will automatically
close and the sealing process will begin.
Once the plate makes contact with the heating platen the display shows the symbol “t” and the countdown of
the programmed sealing time starts.
When the countdown timer reaches zero the drawer automatically opens. The sealed plate can now be removed.
The unit emits an audible beep once the heating platen has reached the programmed temperature again and a
new cycle can be started.
Note: Foil backed lm may remain hot for a number of seconds after sealing. Care should be taken in handling
these plates.
3.5 Operation - "Stand-by" Mode
If left idle for a prolonged time the sealer will automatically switch into stand-by mode (factory set at 60 minutes)
and the temperature of the heating plate will be reduced to 60°C.
When entering into stand-by mode the display shows the decrease of the temperature in real time and the amber
LED is switched off.
The temperature of the heating platen will be maintained at 60°C during standby.
The display shows the actual temperature of the heating platen and not the set sealing temperature.
By pressing any key the unit will automatically begin to warm up the heating plate to the pre-selected sealing
temperature.
Refer to section 4.4 to change the time after which the unit switches into stand-by mode or to switch off the stand-
by function.

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