3.6 "Power-down" Mode
If the unit remains idle in stand-by mode for a prolonged time the system will go into power down mode and the
heating platen will be switched off (factory set at 10 hours). The amber LED is switched off.
The display shows the temperature decrease until it reaches 45°C, after this “LO” will be displayed.
By pressing any key the unit will automatically begin to warm up the heating platen to the pre-selected sealing
temperature.
Refer to section 3.3 to change the time after which the unit switches into power-down mode.
3.7 Closing Drawer
When the unit is not in use, to prevent damage or dust contamination the drawer can be closed by pressing the
“CLOSE” (DOWN) button.
WARNING! Do not close the drawer by hand
4 Optimising Seal Quality
4.1 Plate Requirements
The 4s3™ accommodates plates made from a variety of materials and plate designs. For a complete list of the
acceptable sealing materials, please reder to section 4.3, page 16.
The 4s3™ is designed to accommodate plates which meet the standard established by the Society of Biomolecular
Sciences (SBS). These include but are not limited to deep-well plates, PCR and standard microplates in the 96,
384 and 1536 well formats.
In accessing the suitability of a plate for heat sealing, it is important to look at its design and the quality of
manufacturing. In general terms, it is important for the plate design to have raised rims around each well, giving a
dened sealing ring around each well. When assessing the seal quality, it is important to study a recently removed
seal looking for a regular pattern of sealing rings from one end of the plate to the other.
Incomplete sealing, indicates that the plate is either not perfectly at (or not attened during the sealing process)
or that the plate is not supported adequately in the plate support adapter.
The 4s3™ is designed to be used with any of the following plate types made from polypropylene, polyethylene,
polystyrene, polycarbonate and COC.
• PCR plates
• SBS format shallow well plates (384-well)
• Roche 1536 well plates
• Deep-well plates
• Tube blocks (various)
• Vision Plates™
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