3MP825P Rev1
(3305/3325 Service Manuals)
Page 2 of 2
(Similar to 3MP825)
The disposable sampler tips in the 3MA800
Micro-Sample Kit and the Ease-Eject™
Sampler are specially designed for freezing-
point depression osmometry with the
Advanced Micro-Osmometers, Models 3300
and 3320. The use of any other means of
containing the sample for use in these models
is not recommended. These specialized items
should not be used in conjunction with any
other laboratory procedure.
Calibration
Your 20 µL Sampler has been carefully cali-
brated at our factory and is ready to use.
Should you wish to re-calibrate your 20 µL
Sampler, follow steps 1, 5, 6, and 7 of the
plunger replacement procedure (over).
Operation
Sampler Tip Installation:
Mount one sample tip firmly on the sampler.
Be sure the sampler tip is straight and firmly
seated.
Sampling:
Depress the sampler plunger and insert the
sampler tip into the sample at least 1/4” or 6
mm below the fluid surface. Gently release
the plunger to draw 20 µL of sample into the
sampler tip and remove the sampler from the
sample.
Visually inspect the sample in the sampler tip.
If there are any large voids or bubbles, the
sample must be expelled and another sample
drawn.
Wipe all excess sample from the outside of
the sampler tip with a clean, no-lint, non-
ionic, absorbent paper tissue to remove any
clinging droplets.
The sample should not extend beyond the end
of the sampler tip (F). Blot the sampler tip
with tissue as necessary to remove excess
fluid but be careful not to wick out the sam-
ple. If you are in doubt, leave a slightly con-
cave meniscus (G).
Holding the sampler by the barrel (not the
plunger), insert the sampler tip into the sam-
ple port until it comes to a positive stop.
Do not push the sampler in by the plunger
handle and do not attempt to inject the sample
into the sample port.
Do not remove the sampler until the test has
been completed. The sample osmolality is
measured inside the sampler tip.
Use a fresh sampler tip for each sample.
Sampler Tip Removal:
To dispose of the used sampler tip, remove
the sampler from the instrument and press
down hard enough on the plunger to dislodge
the tip, then discard.
Blot the sampler plunger with a lint-free tis-
sue to avoid contaminating the next sample.
Be careful not to dislodge the teflon plunger
tip (H).
Install a fresh, disposable sampler tip.
Between runs, rinse the plunger with water or
alcohol and wipe dry.
Check the sample size with the calibration
key daily.
F
H
G
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