Exercise 4 – Optimize Acquisition parameters
Task 1. Use Optimizer to optimize acquisition parameters
Agilent 6400 Series Triple Quad LC/MS Familiarization Guide 65
6 Start the optimization process. • Click the Start Optimization button
( ) on the toolbar
or
• Click the Ion Breakdown Profile
button ( ) on the toolbar.
7 Review results. a Click the Compound Setup tab.
b Mark the Show results summary
check box above the table.
c Review the following values for each
transition ion in the Compound Table:
• Fragmentor
• Collision Energy
d Review the printed optimization
report.
• (optional) Use the MassHunter
Workstation Qualitative Analysis
program to look at the data.
• See the online Help for Optimizer or
the Optimizer Quick Start Guide to
learn how to import optimization
results to acquisition for MRM time
segments.
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