Introduction 
24  Agilent Cary 3500 UV-Vis Spectrophotometer User’s Guide 
Compressed gas purging 
The sample compartment of the Cary 3500 UV-Vis can be nitrogen or compressed 
air purged if the purge tubing is fitted. The gas supply should be attached to the 
inlet tube on the back of the module. 
You may need to purge the instrument with nitrogen or compressed air under the 
following circumstances: 
  When using temperatures lower than the ambient temperature to prevent 
condensation in the sample compartment 
  When operational conditions are such that environmental vapors or solid 
particles could cause damage to optical surfaces. 
Purge kit 
The Cary 3500 UV-Vis Purge kit, G9891A, includes the 0-30 LPM flow meter and 5 
meters of 6 mm OD/4 mm ID tubing. See the Cary UV Workstation Help and 
Learning Center for instructions on how to install the purge kit.  
Table 4  Purge kit pressure requirements 
Cary 3500 UV-Vis Purge Kit 
 
Pressure regulator and gauge 
The operating pressure of the Agilent supplied gauge is up to a maximum of 
689 kPa (100 PSIG). Always use an appropriate regulator and gauge to ensure that 
the purge gas supply is consistently maintained at the correct pressure. If a 
different flow meter is used, then the maximum operating temperature will be 
different. Refer to the manufacturers advice. 
Supply tubing 
Use clean, flexible plastic (polyvinyl chloride (PVC) or equivalent) 6 mm OD/4 mm ID 
tubing.  
Never use rubber tubing, because it may be treated internally with talc that can 
be blown into the optical system.