6 | Operation
Büchi Labortechnik AG
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Operation Manual Pure Chromatography Instruments
u Tap the On/Off switch.
ð The instrument is starting up.
6.6.2 Selecting the flash mode (Pure C-850 only)
Navigation path
➔ Mode
u Navigate to the
Mode
menu according to the navigation path.
u Select [Flash].
ð The flash mode is selected.
6.6.3 Starting Flash separation manually
Precondition:
R The instrument is prepared. See Chapter6.6.1 "Preparing the instrument",
page63
R The required solvents are connected and assigned. See Chapter5.7 "Assigning
solvent to solvent lines", page26
R The sample is prepared.
R The waste bottle is empty.
u Calibrate the solvent level sensor. See Chapter5.8 "Calibrating the solvent level
sensor", page27
u Calibrate the waste level sensor. See Chapter5.12 "Calibrating the waste level
sensor", page29
u Open the protection shield.
u Place the fraction collection trays inside the instrument. See Chapter6.4.5
"Inserting the fraction collection tray", page60
u Close the protection shield.
u Select a cartridge. See Chapter6.2.1 "Selecting a cartridge (Flash mode)", page42
u Tap the drop-down list next to A: on the
Solvent Selection
panel.
ð The display shows a drop-down list with the assigned solvents.
u Tap the required solvent.
ð The solvent is selected
ð The drop-down list closes.
u Select more solvents for the mobile phase according to your needs.
u Edit the gradient according to your needs. See Chapter6.2.4 "Editing the
gradient", page45
u Select the sample collection in the
Collection
panel. See Chapter6.2.7 "Editing
the fraction collection criteria", page49
u Select the collection criteria in the
Collection criteria
panel.
u Tap the button [Start] on the
Run
panel.
ð The display shows the dialog box
Sample Loading
.
u According the requirements adjust the settings. See Chapter6.4.7 "Selecting
values on the dialog box Sample Loading", page61
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