Buchi Pure C-810 - Performing Prep Separations

6 | Operation
Büchi Labortechnik AG
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Operation Manual Pure Chromatography Instruments
6.7 Performing a Prep separation using a method
NOTE
Pure C-830 / C-835 / C-850 only
6.7.1 Preparing the instrument
Time required: approx. 30 sec.
Precondition:
R All commissioning operations have been completed. See Chapter5 "Installation",
page24
u Switch the On/Off master switch to On.
u Tap the On/Off switch.
ð The instrument is starting up.
6.7.2 Selecting the prep mode (Pure C-850 only)
Navigation path
➔ Mode
u Navigate to the Mode menu according to the navigation path.
u Select [Preparative].
ð The prep mode is selected.
6.7.3 Starting a Prep separation using a method
Precondition:
R The instrument is prepared. See Chapter6.7.1 "Preparing the instrument",
page66
R The required solvents are connected and assigned. See Chapter5.7 "Assigning
solvent to solvent lines", page26
R The sample is prepared.
R The waste bottle is empty.
u Calibrate the solvent level sensor. See Chapter5.8 "Calibrating the solvent level
sensor", page27
u Calibrate the waste level sensor. See Chapter5.12 "Calibrating the waste level
sensor", page29
u Open the protection shield.
u Place the fraction collection trays inside the instrument. See Chapter6.4.5
"Inserting the fraction collection tray", page60
u Close the protection shield.
u Open an existing method. See Chapter6.4.6 "Selecting an existing method",
page61
u Tap the button [OK].
u Tap the button [Start] on the Run panel.
u According the requirements adjust the file name.
u Tap the button [OK].
ð The display shows the dialog box Sample Loading .
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