Buchi Pure C-815 - Page 69

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Operation | 6
Operation Manual Pure Chromatography Instruments
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u Select the sample collection in the Collection panel. See Chapter6.2.7 "Editing
the fraction collection criteria", page49
u Select the collection criteria in the Collection criteria panel.
u Tap the button [Start] on the Run panel.
ð The display shows the dialog box Sample Loading .
ð The instrument starts the separation.
u According the requirements adjust the settings. See Chapter6.4.7 "Selecting
values on the dialog box Sample Loading", page61
u Follow the instructions on the display.
ð Installing the column. See Chapter6.4.4 "Installing and removing a column",
page58
ð Introducing the sample into the system. See Chapter6.4.1 "Introducing a sample
into the prep system", page54
6.8.4 Changings during a separation
NOTE
Parameters that can be edited are highlighted in green
Possibilities to edit the gradient during a separation:
 Change the gradient. See Chapter "Editing the gradient in graphic mode", page47
 Hold the gradient. See Chapter6.1.6 "Gradient panel", page39
 Auto gradient hold. See Chapter6.1.6 "Gradient panel", page39
6.8.5 Ending a Prep separation
NOTE
The separation extends automatically by 5 minutes if the baseline at the end of the
separation is not below the set threshold.
Precondition:
R The display shows the dialog box Separation End .
u According to the requirements extend the separation time by 5 minutes.
6.8.6 Shutting down the instrument
Navigation path
➔ File
Precondition:
R The separation process has ended.
u Removing the column. See Chapter6.4.4 "Installing and removing a column",
page58
u Purge the instrument with purging solvent. See Chapter7.6 "Cleaning the
instrument", page77
u Navigate to the [File] menu via the navigation path.
u Tap the action [Shut down].
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