Buchi Pure C-815 - Selecting the Prep Mode (Pure C-850 Only)

6 | Operation
Büchi Labortechnik AG
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Operation Manual Pure Chromatography Instruments
6.8.1 Preparing the instrument
Time required: approx. 30 sec.
Precondition:
R All commissioning operations have been completed. See Chapter5 "Installation",
page24
u Switch the On/Off master switch to On.
u Tap the On/Off switch.
ð The instrument is starting up.
6.8.2 Selecting the prep mode (Pure C-850 only)
Navigation path
➔ Mode
u Navigate to the Mode menu according to the navigation path.
u Select [Preparative].
ð The prep mode is selected.
6.8.3 Starting a separation
Precondition:
R The instrument is prepared. See Chapter6.8.1 "Preparing the instrument",
page68
R The required solvents are connected and assigned. See Chapter5.7 "Assigning
solvent to solvent lines", page26
R The sample is prepared.
R The waste bottle is empty.
u Calibrate the solvent level sensor. See Chapter5.8 "Calibrating the solvent level
sensor", page27
u Calibrate the waste level sensor. See Chapter5.12 "Calibrating the waste level
sensor", page29
u Open the protection shield.
u Place the fraction collection trays inside the instrument. See Chapter6.4.5
"Inserting the fraction collection tray", page60
u Close the protection shield.
u Install a column. See Chapter6.4.4 "Installing and removing a column", page58
u Tap the drop-down list next to A: on the Solvent Selection panel.
ð The display shows a drop-down list with the assigned solvents.
u Tap the required solvent.
ð The solvent is selected
ð The drop-down list closes.
u Select more solvents for the mobile phase according to your needs.
u Edit the gradient according to your needs. See Chapter6.2.4 "Editing the
gradient", page45
u Enter the required times in the Conditions panel.
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