Class II Biological Safety Cabinets
o 1N Hydrochloric Acid
o 1N Sodium Hydroxide
o 1% Quaternary Ammonium Compound
o 5% Formaldehyde
o 5,000 ppm Hypochlorite
o 2% Iodophor
o 5% Phenol
o 70% Ethyl Alcohol
• Adequate contact time should be observed for effective decontamination and the time required
depends on the disinfectant agents, the concentration and the object of disinfection.
• There is no one disinfectant agent that works with all organisms. Therefore, users and safety
professionals should carry out risk assessment to ensure that appropriate disinfectant agents and
validated decontamination procedures are used in decontaminating the BSC.
3.7. Gaseous Decontamination
Decontamination may frequently be carried out by means of formaldehyde fumigation or using other
decontamination agents, such as chlorine dioxide or hydrogen peroxide. Decontamination process should only
be carried out by qualified personnel.
In any of the following eventualities, the user should ensure that the BSC has been properly decontaminated,
keeping in mind the nature of the pathogens used:
• At the time of moving/relocating the BSC
• At the time of changing the type of work being carried out in the BSC
• Before accessing contaminated areas for servicing (e.g. when filter needs replacement)
• Periodically and as mandated by your risk assessment
3.8. Further Information
• A Guide to Biosafety and Biological Safety Cabinets can be downloaded from
http://escoglobal.com/resource.php?id=13
• An educational video on “Working Safely in your Biological Safety Cabinet” is available for viewing at
http://www.youtube.com/watch?v=ZnUW1N-JJz8
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