Access Array System for the Ion Torrent PGM Sequencing System: User Guide
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Chapter 3: Target-Specific Primer
Validation for 4-Primer Amplicon Tagging
on the LP 48.48 IFC
This chapter describes the validation procedure for the tagged TS primers designed in
Chapter 2.
IMPORTANT Access Array
™ Barcode 1 primers for the Ion Torrent™ PGM™ Sequencer - 96 must
be ordered prior to primer validation. See Table 1 for oligo sequences and order through an
oligonucleotide vendor.
Reference Documents
Agilent® DNA 1000 Kit Guide
Materials
Required Reagents
IMPORTANT
• This guide is for preparing sequencing libraries with Access Array reagents. To prepare
targeted DNA sequencing libraries with Advanta NGS Library Reagent Kits, see the
Advanta NGS Library Preparation with Access Array Protocol (PN 101-7885).
• Store reagents according to manufacturer's storage recommendations as soon as they are
received.
Reagents Supplied by Fluidigm
Product Name Manufacturer Part Number
20X Access Array Loading Reagent Fluidigm 100-0883
Access Array Barcode 1 primers for Ion Torrent with CS1/CS2 tags Fluidigm —
Target-specific primer pairs tagged with universal tags (CS1 forward tag,
CS2 reverse tag)
50 M CS1–tagged TS forward primer
50 M CS2–tagged TS reverse primer
Fluidigm —
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