Fluidigm Access Array - Prime the IFC

Access Array System for the Ion Torrent PGM Sequencing System: User Guide
Chapter5:Bidirectional Amplicon Tagging on the LP 48.48 IFC
Prime the IFC
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• 1X Access Array Hydration Reagent v2 (Fluidigm, PN 100-7966)
IMPORTANT This reagent is not required, but it ensures uniform harvest volumes if
used
during the priming step when using the Access Array 48.48 IFC. (It is not for use with
the LP 48.48 IFC.)
• Access Array Barcode Library for Ion Torrent PGM Sequencer - 96 (Fluidigm,
PN 100-4911)
• Target-specific primer pair with universal tags (CS1 forward, CS2 reverse), supplied in
separate forward and reverse primer pools in 96-well plates at the following stock
concentrations:
• 50 M CS1-tagged TS forward primer
• 50 M CS2-tagged TS reverse primer
• Template DNA at 50ng/L
Stored at 4 ºC
Agilent DNA 1000 Kit Reagents (Agilent, PN 5067-1504)
Stored at Room Temperature
PCR Certified Water (Teknova, PN W3330)
Prime the IFC
NOTE The location of the sample inlets is different from 48.48 Gene Expression or
Genotyping IFCs.
Accumulators
H1
H2
H3
Sample
Primer
inlets
inlets
H4