Fluidigm Access Array - Appendix B: Access Array Barcode Library for the Ion Torrent PGM Sequencer

Access Array System for the Ion Torrent PGM Sequencing System: User Guide
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Appendix B: Access Array Barcode
Library for the Ion Torrent PGM
Sequencer - 96
The Access Array™ Barcode Library for the Ion Torrent™ PGM™ Sequencer - 96 (Fluidigm,
PN 100-4911) is designed to be used for bidirectional sequencing strategy on the Ion Torrent
sequencer for amplicon libraries that have been generated on the Access Array™ system.
Table 14. Access Array Barcode Library for Ion Torrent PGM Sequencer - 96
Table 15. Barcode Sequences for Access Array Barcode Library for Ion Torrent PGM Sequencer - 96
(Fluidigm, PN100-4911)
PN Plate
Number
Reagent Description Volume
(L)
100-4912 A1 Access Array Barcode Library for Ion Torrent PGM Sequencer P1_CS2 /
A_BC_CS1_1-96 at 2 M per oligo
50
100-4913 B1 Access Array Barcode Library for Ion Torrent PGM Sequencer P1_CS1 /
A_BC_CS2_1-96 at 2 M per oligo
50
Barcode Name Barcode Sequence Barcode Well Plate
MID-1 ACGAGTGCGT A1 A1 & B1
MID-2 ACGCTCGACA B1 A1 & B1
MID-3 AGACGCACTC C1 A1 & B1
MID-4 AGCACTGTAG D1 A1 & B1
MID-5 ATCAGACACG E1 A1 & B1
MID-6 ATATCGCGAG F1 A1 & B1
MID-7 CGTGTCTCTA G1 A1 & B1
MID-8 CTCGCGTGTC H1 A1 & B1
MID-10 TCTCTATGCG A2 A1 & B1
MID-11 TGATACGTCT B2 A1 & B1
MID-13 CATAGTAGTG C2 A1 & B1
MID-14 CGAGAGATAC D2 A1 & B1
MID-15 ATACGACGTA E2 A1 & B1