Access Array System for the Ion Torrent PGM Sequencing System: User Guide
Chapter 5: Bidirectional Amplicon Tagging on the LP 48.48 IFC
Check PCR Products on the Agilent 2100 Bioanalyzer
35
Check PCR Products on the Agilent 2100 Bioanalyzer
1 Use the Agilent DNA 1000 chips from the Agilent DNA 1000 Kit to check 1 L of PCR
product from each of the PCR reactions described above. Follow the Agilent DNA 1000
Kit Guide for details.
2 Check the results of the chip to determine if the PCR product pool has the expected size.
Depending on the expected sizes of the PCR products, a smear may be visible.
Comparison of the electropherogram to a histogram of the expected sizes indicates
correctness of the product size range. The PCR products of the barcoding step should
exhibit a band shift of +63 bp when compared to Harvest Pool Products.
3 Store the PCR products at –20 ºC.
Next Step
Continue with your library preparation as described in Post-PCR Amplicon Purification and
Quantitation on page 36.
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