Fluidigm Access Array - Load the IFC

Access Array System for the Ion Torrent PGM Sequencing System: User Guide
Chapter5:Bidirectional Amplicon Tagging on the LP 48.48 IFC
Load the IFC
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2 Vortex the sample mix solution for a minimum of 20 seconds and centrifuge for
30 seconds.
IMPORTANT It is essential to vortex all components to ensure complete mixing.
Load the IFC
1 Pipet 4 L of 20X primer solution into each of the primer inlets.
2 Pipet 4 L of sample mix solution into each of the sample inlets.
IMPORTANT While pipetting, do not go past the first stop on the pipette. Doing so may
introduce air bubbles into the inlets.
NOTE An 8-channel pipette is recommended to load the sample mix and 20X primer
solutions. The recommended pipetting order is shown below.
3 Load the IFC into the pre-PCR IFC Controller AX in the pre-PCR lab.
4 Press Eject to move the tray out of the IFC Controller AX.
5 Place the IFC onto the tray by aligning the notched corner of the IFC to the A1 mark.
6 Press Load Chip to register the barcode of the IFC and activate the script selection.
7 Load the LP 48.48 IFC for Access Array. Select Load Mix (155x) and Run Script.
NOTE For Access Array 48.48 IFCs, select Load Mix v7 (151x) and Run Script. Load Mix
v7 (151x) is a script update from Load Mix (151x) for Access Array 48.48 IFCs and can be
downloaded from fluidigm.com/software. Contact Technical Support for assistance.
8 Once the script is complete, press Eject to remove the IFC.
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