GE akta start User Manual

ÄKTA™ start
System Cue Card
Contents
System overview 2
System preparation
4
Column preparation
6
Starting a run
6
Monitoring a run
7
Quick start methods
8
Flow charts 1
0
Getting help 1
1
Reference information 1
1
System Overview
ÄKTA start is a simple and modern liquid chromatography system intended for
preparative purication of proteins at laboratory scale.
The system can be used for a variety of research purposes to fulll the needs of the
users in the academia and in the life sciences industry. ÄKTA start is intended for
research use only, and should not be used in any clinical procedures, or for diagnostic
purposes.
The System Cue Card describes how to prepare the system for a chromatography run,
start and monitor a run, and evaluate the result.

GE akta start Specifications

General

UV Detection Wavelength Range	190 to 700 nm
pH Detection Range	2 to 12
Computer Interface	USB, Ethernet
Software	UNICORN
Weight	15 kg
Type	Chromatography system
Applications	Protein purification
Conductivity Detection Range	0.1 to 999.9 mS/cm
Operating Temperature Range	4 to 40 °C
Power Requirements	100-240 V AC, 50/60 Hz

Summary

System Overview

Instrument Illustration

Shows the physical layout and identifies key modules of the ÄKTA start system.

Instrument Display - Home Screen

Details the four main options available on the ÄKTA start's home screen interface.

Frac30 Fraction Collector and UNICORN Software

UNICORN Start Overview

Explains the UNICORN start software package for instrument control and method editing.

Safety Precautions

Highlights critical safety information for operating the instrument.

System Preparation and Setup

Preparation Workflow

Step-by-step instructions for preparing the ÄKTA start system.

System Connectivity

Guide on connecting the PC and Frac30 to the ÄKTA start instrument.

Fractionation and Calibration

Procedures for configuring fractionation and calibrating instrument modules.

Flow Path and Sample Handling

Prefill the Flow Path

Instructions for cleaning and filling the system's flow path.

Connect a Column

Steps for attaching and connecting a chromatography column.

Sample Application

Methods for applying sample to the column via pump or loop.

Cold Room Operations

Procedures for operating the instrument in a cold room environment.

Starting and Performing Runs

Perform a Method Run

Workflows for executing chromatography runs using predefined methods.

Perform a Manual Run

Workflows for manually controlling and running the system.

Column Preparation

Methods for preparing new columns or equilibrating existing ones.

Monitoring and Controlling Runs

Monitor from Instrument Display

Options for monitoring runs directly on the ÄKTA start display.

Monitor from UNICORN Start

How to view and control runs using the UNICORN start software.

Evaluating and Managing Results

Save Results to USB

Steps to save run results onto a USB memory stick.

Import Results to UNICORN

How to import run data into UNICORN start for analysis.

Quick Start Methods

Introduction to ready-to-run methods for common purification techniques.

Quick Start Method Details

Affinity Chromatography (AC) Quick Start

Details and parameters for Affinity Chromatography quick start methods.

Desalting/Buffer Exchange (DS) Quick Start

Details and parameters for Desalting/Buffer Exchange quick start methods.

Flow Charts and Diagrams

Detailed Flow Path Diagrams

Visual representations of system flow paths during various operations.

User Support and Resources

Instrument and Software Help

Accessing help features within the instrument display and UNICORN software.

Ordering and Selection Guides

Information on ordering products and selecting appropriate columns and buffers.

Buffer Selection and Documentation

Buffer Suggestions by Type

Recommended buffer types and specific suggestions for AC, DS, IEX, and GF.

Health and Safety Declaration Form

Information regarding the Health & Safety Declaration Form.

User Documentation Reference

A list of available user documentation for the ÄKTA start system.