Germfree BBF 2 - Performance Testing

Class II, Type A2 Biosafety Cabinet
User Manual
13
Performance Testing
Your Class II, Type A2 Biosafety Cabinet features a one piece welded construction. No access panels are required
and it has no jointed segments that might loosen during shipment. This is a distinct advantage because many
potential sources of the leaks through gaskets are avoided.
Your Class II, Type A2 Biosafety Cabinet has been thoroughly tested at the factory. To insure that the supply
and exhaust HEPA filters were free of defects; Poly Alpha Olefin (PAO) or an equivalent was aerosolized into
the unit on the upstream side of the HEPA filters. Integrity of the HEPA filters was determined by scanning
with a photometer to detect PAO or equivalent aerosol particles on the downstream side of the HEPA filters. If
there was any penetration greater than .01% through the filter medium or perimeter filter seal, the leaks were
located and repaired. Air velocity was measured with a thermoanemometer to insure that it was uniform and
unidirectional. Other tests for noise level, vibration level and light intensity were performed; the Class II, Type
A2 Biosafety Cabinet meets or exceeds recommended standards. A simple test of the air balance which you can
perform aer the unit is set up is carried out with a smoke generator. The smoke source is held near the access
port of the work area. If the unit is working properly and is unobstructed, the air curtain will direct the smoke
downward. No smoke should enter the work area or flow over the work platform, all air should flow downward
within the unit and no air should exit the interior.