Class II, Type A2 Biosafety Cabinet
User Manual
15
with open cuffs allow the entrapment of contaminated air around the technician’s arms inside the sleeves. The
hands and arms should be inserted into and withdrawn from the work area slowly to prevent “dragging” of
contaminants from the room into the work area or from the work area into the room. Abrupt movements of the
arms and hands should be avoided. Excessive activity in the room may also create disruptive air currents. This
should be held to a minimum or eliminated when work is being performed in the unit. The viewing panel should
never be opened while work is in progress. When the work has been completed, cover the tray for the discarded
supplies with a towel or paper towels soaked in an appropriate germicide. The outside of the tray and other
equipment (glassware, etc.) can be wiped or sprayed with the appropriate solution. Wait approximately five
minutes before removing any item from the chamber.
Biological Safety Cabinet Operation “Don’ts”
1. DON’T ever cover the air intake area in front of the chamber with papers, notebooks, equipment, etc.
Movement of air into the negative pressure section must not be impeded!
2. DON’T ever cover the exhaust filter.
3. DON’T ever attempt any maintenance of the motor or any component outside the work area without
decontaminating the unit.
4. DON’T overload the work area.
5. DON’T carry out procedures in front of the work tray, e.g., too near the air curtain.
6. DON’T place items in a position that would interrupt the laminar flow pattern.
7. DON’T introduce or remove your hands rapidly from the unit.
8. DON’T allow spilled viable material to remain in your BBF.
9. DON’T move contaminated items over clean items.
10. DON’T work with open cuffs or other loose garments that might entrap viable agents.
11. DON’T allow trash to get under the work tray.
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