Single Wavelength Mode
5940018SingleWave.fm Page 47
Section 7 Single Wavelength Mode
The Single Wavelength Mode can be used in three ways. For a known parameter
wavelength, set the instrument to measure the absorbance, % transmittance, or
concentration of the analyte.
Percent (%) transmittance measures the percent of the original light that passes
through the sample and reaches the detector.
Absorbance measures the amount of light absorbed by the sample, in units of
Absorbance.
Turning on the concentration factor allows selection of a specific multiplier for
converting absorbance readings to concentration. In a graph of concentration
versus the absorbance, the concentration factor is the slope of the line.
7.1 Setting Up Single Wavelength Mode
In Single Wavelength reading mode, the instrument will begin reporting a
concentration value. Touch
Abs/%T to toggle through the various
reading modes.
7.2 Taking Single Wavelength Measurements
1. Prepare a blank.
2. Place the sample cell containing the blank in the cell holder. Touch
Zero.
3. Remove the blank from the cell compartment. Place the sample cell in the cell
holder. Read the results. (The reading will appear within 20 seconds.)
1. From the Main Menu, touch
Single Wavelength.
2. Look at the second button from the top on the vertical menu bar.
Touch
%T to switch to % transmittance readings. Touch Abs to
switch to absorbance readings. Touch
Conc to switch to
concentration, if it is enabled (see step 5).
3. Touch λ on the right menu bar to change the wavelength. Key in
the desired wavelength and touch
OK.
4. Touch Options to access store, send, or recall data, turn on the
concentration mode, and set the desired resolution for
concentration readings.
5. To convert absorbance readings to concentration, touch
Concentration Factor. Touch On to turn on this feature. Touch
Factor to enter the factor by which absorbance readings are to be
multiplied. Touch
Units to select the units for concentration
measurements. Touch
OK.
6. To change the number of decimal places shown in the
Concentration reading, touch
Concentration Resolution and
make a selection.
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