29
4) Look through the underside of the slide while adjusting the tissue so desired margin or other aspect
is visible in position.
5) Let sample protrude from the end of the slide and touch the leading edge of the tissue to the mould
floor. It will adhere.
6) Pull the dispensing slide out from under the tissue. Tissue requiring precise positioning can be
flattened or manipulated and guided into desired position by carrying out this step and carefully.
7) Fill the mould as quick as possible so that a meniscus of medium is bulging above the brim. Too
much medium is not a problem. Too little medium can cause failure of the specimen to anchor to the
chuck
To Next Page
Loading...