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A.5. Skin sample for MOHS with ring technique
The difficult part in skin sample freezing is to put it with all its margins in the same level and so in one slide.
Milestone studied a method that, through the use of a planar and cold surface, allows to fix all the margins to
the flat plate, and then thanks to the ring, allows the inclusion of the leveled margins sample.
1) Remove from the PrestoCHILL working area the heat extractors and the six moulds, unscrewing
them. Insert the pad and then the ring plate with the serigraphy down. Add the needed tools.
2) Relax and ink the sample following lab procedures.
3) Clean plate and ring, using ethanol moistened paper, just before to use them.
4) Put the sample on the cleaned plate. Press all the lateral margins with tweezers or fingers, in order
to adhere them to the plate. The absence of the mould allow an easier manipulation of the sample.
Before to freeze the sample on the plate, check that the position of the sample allows to put
the chosen ring around it.
The space needed for the ring is:
- 20mm for the RNG7037-5
- 25mm for the RNG7028-5.
Don’t put samples on the external border of the plate.
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