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A. Appendix – Tips and Tricks ................................................................................................................ 50
A.1. Fat tissues........................................................................................................................................ 50
A.2. Muscle tissue ................................................................................................................................... 52
A.3. Small Biopsies ................................................................................................................................. 53
A.4. Skin sample for MOHS with glass disc technique ........................................................................... 55
A.5. Skin sample for MOHS with ring technique ..................................................................................... 56
A.6. Freezing of high sample in vertical position ..................................................................................... 58
B. Appendix – Freezing time ................................................................................................................... 61
B.1. Recommended freezing time for moulds ......................................................................................... 61
B.2. Freezing time changes .................................................................................................................... 62
C. Appendix – Use the extractor for chucks ............................................................................................ 63
C.1. How to use the extractor for chucks ................................................................................................ 63
D. Appendix – How to change a mould ................................................................................................... 65
D.1. How to change a mould ................................................................................................................... 65
E. Appendix – Troubleshooting ............................................................................................................... 66
E.1. Block detachment from the chuck ................................................................................................... 66
E.2. Gap between sample and medium .................................................................................................. 67
E.3. Block sticking to the mould .............................................................................................................. 67
E.4. Difficulty in unscrewing moulds ....................................................................................................... 68
E.5. Power failure .................................................................................................................................... 69
F. Appendix – How to manage an alarm................................................................................................. 70
F.1. How to manage an alarm ................................................................................................................. 70
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