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9) After the 1 minute re-cooling, attach the chuck in its cryostat housing in the same position used for
the trimming.
10) Cut immediately in order to get a slice before the warming up of the block. It is very important to cut
the block in quickly, moving the cryostat wheel very fast.
11) If, during cut, the section curls up, flatten it using two small brushes.
12) Pick up the section and fix immediately.
It is possible to repeat the cooling down of the trimmed block as needed to obtain a good frozen slice.
Milestone suggests the use of MCC medium for better adhesion between the sample and embedding
medium. Thanks to its higher fluidity, MCC flows faster to surround and adhere to the samples.
If some non-fatty parts are present in the sample and they shatter, it is possible to warm these parts up with
gloved thumb.
Last problem about fat is that this material tends to adhere less to the glass and then to came off during
staining. In this case, it is possible to try warming the glass microscope slide before picking up the slice or
cut a thinner slice.
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