Operation Manual Applications
11-2
Processing Difficult Cells
When processing difficult cells, the use of enzymes should be considered. Glusulase can be used with
yeast, lysostaphin can be used with staphylococcus, collagenase can be used with skin and cartilage,
and trypsin hyaluronidase can be used with liver and kidney.
Glass beads in the 10 to 50 micron size range can also be added to the liquid to expedite processing.
When using glass beads, a good ratio is beads, liquid. Glass beads concentrate the energy
released by the implosion and facilitate the crushing of cell walls. After processing, the glass will sink
to the bottom of the vessel or can be centrifuged or filtered out.
Note: For further application information see section 15 (Literature Request).
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