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● If the slider annular ring image is diverged from the phase plate image in the objective, a low
phase contrast image will result.
● For phase contrast microscopy at the maximum contrast, use GIF (Green interference filter) in
the optical path.
V. Photomicrographic Procedure (Model AE21 Only)
● The optical path selector lever can be used to set the optical path to either the Binocular tube
100:0 or Binocular tube / vertical tube 20:80 (observation: photo).
● Before starting photomicrography, check the following:
● The condenser is centered.
● The condenser annular diaphragm is centred.
● The field of view diaphragm is stopped down to slightly just outside the edge of the field of view.
● For photomicrographic procedures, refer to the manual of the specific camera being used.
Filter selection
Filter holder holds up to two filters
Filter type Procedure
Diffuser To be used in all cases
GIF (Green interference) 546nm
For phase contrast microscopy and for
improving the contrast in monochrome
photomicrography
NCB (Neutral Colour Balance) Blue
For routine microscopy and colour
photomicrography
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