7
7. Mounting the Eyepieces
● Remove the dust caps from the eyepiece tubes.
● Insert the eyepieces into the eyepiece tubes and tighten the eyepiece clamp screws.
● Be sure to use eyepieces with the same magnification.
● If the rubber eye guards are to be used, fit them in the groove around the eyepiece.
IV. Microscopic Procedure
1. Interpupillary Distance Adjustment
● Before adjusting the interpupillary distance, bring a specimen into focus using the 10x
objective.
● Adjust the interpupillary distance so that both the right and left field of view become one.
● This adjustment will enable the user to observe the specimen with both eyes.
2. Diopter Adjustment
● Diopter adjustment compensates for the differences in vision between the left and right eyes. In
addition to making observation through both eyes easier, this adjustment also reduces the
extent to which focusing is lost when the objective magnification is changed. In particular, this
occurs when a low magnification objective is used.
● The left eyepiece has a separate focusing provision to compensate for slight differences in the
focusing of each eye.
● Using the right eye only and viewing through the right-hand eyepiece, adjust the focus with the
microscope fine or coarse adjustment until the image of the specimen is at its sharpest.
● Using the left eye only and viewing through the left-hand eyepiece with its independent
diopter-focusing ring, focus until the specimen image is at its sharpest.
● The microscope should now be ready binocular viewing.
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