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START-UP AND OPERATION
• Immersion objectives
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Created by Oleksandr Panasovskyi
40x
100x/Oil
Illustration 020: The 100x oil-immersion objective.
The oil immersion can only be done with a suitable 100x objective!
The 100x oil objective is included in the scope of delivery.
Cedar oil is included in the delivery. We recommend oil with a
refractive index (RI) nD=1.515 according to ISO 8036.
Focus on the object with the 40x objective.
Turn the 40x objective sideways.
Put a drop of immersion oil (1) onto the cover glass of the specimen slide.
Turn in the 100x objective, the front lens is immersed in the oil.
Slowly refocus the image.
Because air bubbles in the oil will affect the image quality, make sure that the oil
drop is free of bubbles. To remove the bubbles, repeatedly defocus and refocus
the oil immersion objective or switch to another objective and let visible bubbles
burst before immersing the 100x objective again.
Clean the 100x objective by dabbing off the oil with a soft baby paper
tissue (lotion-free). When the immersion lens is used regularly, wet clean the lens
only once a week, Use a solution of 70% ethanol or Isopropanol (use p.a. or phar-
macy grade alcohol and double distilled water to make such a solution).
If immersion oil comes into contact with the skin, rinse thoroughly with soap and
water. If immersion oil gets into your eyes, rinse under running water for at least 15
minutes.
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