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Nexcope NE620T - Trouble Shooting Guide; Optical System

Nexcope NE620T
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56
7. TROUBLE SHOOTING GUIDE
Under certain conditions performance of the unit may be adversely affected by factors other than
defects. If problems occur, please review the following list and take action as needed. If you can-
not solve the problem after checking the entire list, please contact Bresser GmbH for assistance.
7.1. Optical system
PROBLEM CAUSE SOLUTION
The edge of the field of view
is dark or the brightness is not
uniform
The nosepiece is not in the located
position (objective and light path not
coaxial)
Check that the nosepiece is locked
in place
The phase contrast slider is not
located in the proper position
Check that the phase contrast slider
is locked in place
A lens (the objective, condenser,
eyepiece or collector) is dirty.
Clean all components thoroughly
Dirt or dust is visible in the
field of view
A lens (the objective, condenser,
eyepiece or collector) is dirty.
Clean all components thoroughly
Dirt/dust on the specimen Clean it thoroughly
The condenser is too low Adjust the height of condenser
Visibility is poor Image is
not sharp; Contrast is poor;
Details are indistinct
Specimen is not covered Place a cover glass on the object
The thickness of the cover glass is
not suitable
Use standard cover glass with
thickness of 0.17 mm
Specimen is placed reversely Place the slide on the stage with the
cover glass facing up
Dry objective has oil on it (especially
for 40x objective)
Clean it thoroughly
A lens (the objective, condenser,
eyepiece or collector) is dirty.
Clean all components thoroughly
Water/immersion oil is not used with
the 100x objective
Use water/immersion oil for the oil
objective
Bubbles are visible in the water or
immersion oil
Remove bubbles in the water/
immersion oil
Immersion oil is unspecified Use specified oil
The aperture iris diaphragm is
stopped down too far
Adjust the aperture iris diaphragm
properly
Dirt or dust on the eyepiece Clean it thoroughly
Diaphragm of the phase contrast
slider is not centered
Center the diaphragm of the phase
contrast slider
The objective used is not fit to the
phase contrast observation
Use the appropriate objective
The diaphragm of phase contrast
slider is not coincident with the
objective phase ring
Adjust the diaphragm to match the
objective phase ring
The condenser is too low Adjust the height of the condenser
7
TROUBLE SHOOTING GUIDE

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