43
VARIOUS OBSERVATION METHODS
5
13 12
11
14
F
ALLEN WRENCH
Illustration 031 F-G: Settings for phase contrast: Centering the phase rings.
Centering of the phase rings
The phase rings have been cen-
tered by the manufacturer. It is rec-
ommended to check the centering be-
fore the phase contrast observation.
Swivel the 10x phase contrast objective into
the beam path.
Set the phase contrast slider to position
10/20/40 and turn the phase ring dia-
phragm to the corresponding position 10.
Focus the specimen.
Unscrew an eyepiece (11) from the binoc-
ular attachment (12) using a suitable Allen
key (13).
If available, then insert the centering tele-
scope (CT) (14) into the socket and turn it up
until two sharp phase rings become visible.
The CT enlarges the phase rings
and makes centering easier. An
approximate centering of the phase
rings is also possible without CT.
15a 15b
16
G
DARK PHASE RING
BRIGHT RING DIAPHRAGM
Check the centering and overlap of the bright
ring diaphragm (in the condenser) with the
dark phase ring (in the objective).
Center the two phase rings if you do not
see any overlay (15a). Adjust the centering
screws (16) on the phase contrast slider un-
til the bright ring diaphragm and the dark
phase ring completely overlap (15b).
Perfect phase contrast can only be
achieved when the bright ring dia-
phragm and the dark phase ring ex-
actly covers the beam path.
Then switch to the next objective, check the
phase rings for all objectives and adjust if
necessary.
To Next Page
Loading...
