Chapter 1 Microscopy Procedures
7
Chap. 1-1
Microscopy Procedures
Bright-field Microscopy
Notes on controlling the focus knobs
Avoid the following actions, which can cause equipment
malfunctions.
Rotating the right and left focus knobs in opposite directions.
Rotating the coarse focus knob past the limit.
POWER
0.1
0.2
0.3
0.40.6
0.7
0.8
0.9
Don't rotate the knobs in opposite directions!
10
Adjust the diopter. (→See Chapter 2, “4 Adjusting the Diopter” for details)
(1) Turn the diopter adjustment ring on the right and left
eyepieces to align the end face of the diopter
adjustment ring with the line. (This is the diopter
adjustment reference position.)
Reference position for diopter adjustment
(2) Focus on the specimen using the 40x objective.
(3) Bring the 10x (or 4x) objective into the optical path.
(4) Look into the right eyepiece with your right eye and
the left eyepiece with your left eye. Turn the diopter
adjustment ring of each eyepiece to focus on the
specimen. At this point no focus knobs are used.
(5) Repeat Steps (2) through (4) to make sure the focus
has been adjusted properly.
Adjusting the diopter
11
Adjust the interpupillary distance.
Look into both eyepieces and rotate the binocular part to
adjust the binocular part’s opening until the fields of view
for the right and left eyes coincide.
Adjusting interpupillary distance
Tip on adjusting the interpupillary
distance
For easy adjustment, look into the eyepiece as if
you were looking at a distant object.
Line
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