Chapter 1 Microscopy Procedures
6
Chap. 1-1
Microscopy Procedures Bright-field Microscopy
(2) Rotate the stage knob to move the stage and bring
the target into the optical path.
(So that the sample sealed under the cover glass
will be lighted.)
POWER
0.1
0.2
0.3
0.40.6
0.7
0.8
0.9
Bringing the target into the optical path
9
Focus on the specimen. (→See Chapter 2 “2 Focusing on the Specimen (Vertical Stage Movement)” for
details)
(1) When using a trinocular tube or ergonomic tube,
push in the optical path switching lever to distribute
100% of light to the binocular section.
(2) Look into the eyepiece and turn the coarse focus
knob away to raise the stage to the upper limit. From
there, focus on the specimen by lowering the stage.
Switching the optical path 100%
to the binocular part
(3) When the focus was roughly adjusted using the
coarse focus knob, turn the fine focus knob to
accurately adjust the focus.
POWER
0.1
0.2
0.3
0.40.6
0.7
0.8
0.9
Focusing on the specimen
(4) Turn the dia-illumination brightness control knob to
adjust the brightness of the field of view.
TORQUE
CL
P
O
WER
0
90
80
30
20
10
Brightness adjustment
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