Nikon Eclipse Ci-E - Page 40

Chapter 1 Microscopy Procedures
28
Chap. 1-4
Microscopy Procedures Sensitive Polarization Microscopy
3
Set the setting mode switch to the left side (operation mode).
4
Turn on the dia-illumination LED.
5
Lower the condenser slightly from the uppermost position.
6
Fully open the field diaphragm and aperture diaphragm.
7
Bring the 10x objective into the optical path.
8
Place a specimen on the stage, and move the stage to bring the target into view.
9
Remove the analyzer and the polarizer from the optical path.
Pull out the analyzer IN/OUT knob from the analyzer tube
for first-order red compensation to remove the analyzer
from the optical path.
The polarizer unit for first-order red compensation has not
been installed at this point.
Removing the analyzer from the optical path
10
Focus on the specimen. (→See Chapter 2 “2 Focusing on the Specimen (Vertical Stage Movement)” for
details)
11
Adjust the diopter. (→See Chapter 2, “4 Adjusting the Diopter” for details)
12
Adjust the interpupillary distance.
13
Focus and center the condenser. (→See Chapter 2, “5 Focusing and Centering the Condenser” for details)