Nikon Eclipse Ci-E - Page 41

Chapter 1 Microscopy Procedures
29
Microscopy Procedures
Sensitive Polarization Microscopy
Chap. 1-4
Microscopy operation
14
Bring a portion of the specimen where there is no sample into the optical path.
Rotate the stage knob to move the specimen and bring a portion where there is no sample under the cover glass into
the optical path.
15
Bring the analyzer and the polarizer into the optical path.
Push in the analyzer IN/OUT knob into the analyzer tube
for first-order red compensation to bring the analyzer into
the optical path.
Set the polarizer unit for first-order red compensation over
the field lens.
Make sure the orientation mark on the polarizer (roughly)
comes to the front at this point.
Keep the fixing screw of the polarizer unit loosened.
CLAMP
TORQUE
0.1
0.3
0.4
0.5
0.2
0.9
0.8
Bringing the analyzer into the optical path
Installing the polarizer
Slider-type analyzer for first-order red
compensation
Using the C-AS Analyzer Slider for First-order Red
Compensation instead of an analyzer tube for
first-order red compensation can keep the eye
point from rising. To use the C-AS Analyzer Slider
for First-order Red Compensation, insert it into the
analyzer slot of the nosepiece. Use the analyzer
slider as follows:
Push in: The analyzer goes into the optical path.
Pull out: The analyzer is removed from and the
dummy hole goes into the optical path
(empty).
Using D-AS analyzer slider
C-AS analyzer slider
A
nalyzer
IN/OUT knob
Pivo
t
Fixing screw
Orientation
mar
k
Lambda
plate rotation
leve
r