Chapter 4 Troubleshooting
82
Chap. 4
Troubleshooting
Problem Cause Measure
One side of the field of
view (up, down, right, or
left) is not focused.
The image flows (i.e.
becomes asymmetrically
defocused when moving
the focal point)
The specimen is tilted relative to the stage
surface.
Position the specimen in place on the stage.
(→Chapter 1 “1.2 Bright-field Microscopy Procedure
- 8 Place a specimen on the stage, and move the
stage to bring the target into view”)
The stage is tilted.
Attach the stage correctly.
(→Chapter 3, “2 Assembly for Bright-field
Microscopy - 2 Attach the stage”.)
Images in left and right
eyepieces are not
coincident.
Interpupillary adjustment has not been
performed.
Perform interpupillary adjustment.
(→Chapter 1, “1.2 Bright-field Microscopy
Procedure - 11 Adjust the interpupillary distance”.)
Diopter adjustment has not been
performed.
Perform diopter adjustment.
(→Chapter 2 “4 Adjusting the Diopter”)
Eyes become fatigued.
Diopter adjustment has not been
performed.
Perform diopter adjustment.
(→Chapter 2 “4 Adjusting the Diopter”)
Brightness is inadequate.
Adjust the brightness using the brightness control
knob to attain a suitable brightness.
(→Chapter 2, “1 Adjusting the Brightness of a
Diascopic Image”)
The specimen does not
move smoothly.
The specimen holder is not securely-fixed
to the stage.
Fix the holder securely.
(→Chapter 1 “1.2 Bright-field Microscopy Procedure
- 8 Place a specimen on the stage, and move the
stage to bring the target into view”)
Rotating torque of the stage knob is set too
heavy.
Adjust to the appropriate torque weight.
(→Chapter 2, “3.3 Adjusting the Knob Rotation
Torque”.)
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