Chapter 4 Troubleshooting
81
Chap. 4
Troubleshooting
Problem Cause Measure
Lack of visibility around
periphery of field of view.
Illumination is uneven
across the field of view.
The field of view is not
visible.
Parts are attached incorrectly.
Confirm that parts (nosepiece, condenser, etc.)
are correctly attached.
(→Chapter 3, “2 Assembly for Bright-field
Microscopy”)
Movable parts are not seated correctly.
Correctly set the optical path switching lever,
nosepiece, filter cube switchover turret,
condenser turret, and slider, etc. (Move the
part until it clicks.)
A field diaphragm image is not focused on
the specimen surface.
Make sure the condenser is focused and
centered.
(→Chapter 2, “5 Focusing and Centering the
Condenser”.)
Lack of visibility around
periphery of field of view.
Illumination is uneven
across the field of view.
The field of view is not
visible.
The field diaphragm is stopped down too
far.
Open the field diaphragm slightly wider than
the field of view.
(→Chapter 2 “8 Adjusting the Field Diaphragm”)
Incorrect combination of the objective with
the condenser.
Adopt an appropriate combination.
(→Chapter 2 “7 Selecting a Condenser”)
In low magnification microscopy, required
condenser operation was not performed.
Perform the operation such as swinging out or
sliding the condenser.
The lens and specimen are dirty or dusty.
Clean them as appropriate.
(→Chapter 5 “1.1 Cleaning Lenses”)
Out of focus with an
objective of high
magnification.
The specimen is upside down.
Turn up the cover glass and attach it to the
stage.
(→Chapter 1 “1.2 Bright-field Microscopy Procedure
- 8 Place a specimen on the stage, and move the
stage to bring the target into view”)
The thickness of the cover glass is
inadequate.
Attach a cover glass of the specified thickness
(0.17 mm).
A fail-safe device for specimen damage
protection of the objective is pushed in.
Some objective has a stopper to keep the
pushed in state. Turn the tip of the object to
release.
If the objective does not have a stopper, do
not pull because the tip cannot be turned. In
this case, contact your nearest Nikon
representative.
A focal deviation is high
when switching over
objectives.
The objective lens is attached incorrectly.
Screw the objective all the way in.
(→Chapter 3, “2 Assembly for Bright-field
Microscopy - 7 Attach the objective”.)
Diopter adjustment has not been
performed.
Perform diopter adjustment.
(→Chapter 2 “4 Adjusting the Diopter”)
The image is not in
focus although the stage
is raised to the highest
position.
The stage is attached incorrectly.
Attach it correctly.
(→Chapter 3, “2 Assembly for Bright-field
Microscopy - 2 Attach the stage”.)
The refocusing position is set lower than
the focusing position.
Check and reset the setting.
(→Chapter 2, “2.4 Refocusing”.)
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