III. Operation of each part
52
Photomicrography procedure and items to be checked
On photomicrography, it is especially important to obtain appropriate illumination and focus the microscope properly.
First adjust the microscope for usual observation. Then, check and perform the following
1
Perform diopter
adjustment.
For TE2000-E, TE2000-U:
Rotate the diopter adjustment rings so that both the photo-mask and
the specimen is in sharp focus.
For TE2000-S: Switching between 10x and 40x objective, use focus knob and diopter
adjustment rings in turn. (See page 47 for details.)
2
Adjust illumination.
Eliminate uneven
illumination.
Lamp voltage: For color photomicrography, set to the same indication as lamp ratings.
Filters: For color photomicrography, set NCB11 filter in the optical path.
Add color compensation filters if necessary.
Condenser: Perform focusing and centering of the condenser (if T-DH dia-
illuminator 100W is used).
On Ph microscopy, perform annular diaphragm centering.
Aperture diaphragm is to be set to 70 to 80% of objective N.A. on
normal use, and to be opened fully on Ph observation with system
condenser.
Camera: “+2/3” exposure adjustment will have good results when 35mm SLR
camera is attached to the front port.
3
Switch the optical
path
Use the optical path switchover dial, remote control pad, or PC to guide the image to the
port on which the photomicrography equipment is attached.
(If this results in sending 100% light to the specified port, you will need to switch back to
the observation port frequently.)
4 Check the trimming.
For TE2000-E, TE2000-U:
Use the photo-mask.
For TE2000-S: Attach the “mask eyepiece” instead of an eyepiece and check, or look
through the finder on the camera or photomicrography equipment.
5
Focus on the film
surface.
For TE2000-E, TE2000-U:
Turn the focus knob so that both the photo-mask and the specimen
image is in sharp focus. When using 4 to 40x objective, turn the
eyepiece tube turret (not equipped on T-TS eyepiece tube S or T-TI
intermediate tube) to “M” to enlarge the image on eyepiece only, and
focus precisely. (Do not touch the diopter adjustment ring after
turning the eyepiece tube turret to “M”.)
For TE2000-S: Focus on the specimen while looking through the finder on the camera
or photomicrography equipment, or through mask eyepiece.
If objective with correction ring is used, check the settings of the correction ring.
6
Shut out the
extraneous light.
Field diaphragm: Close down to a size slightly larger than the photo frame.
Eyepieces: Turn the eyepiece tube turret (not equipped on T-TS eyepiece tube S
or T-TI intermediate tube) to “C”. Or, cover the eyepieces with a
piece of cloth.
Finder on camera or photomicrography equipment:
Cover the finder with a piece of cloth or a finder cap. (Always leave
the cover on, unless required to look into the finder.)
7
Eliminate the
influence of
vibration.
If exposure time is shorter than “1/8s”, insert ND filters in the optical path to make the
time longer than “1/8s”.
(If monochromatic film is used, you can also use lamp voltage to darken the image.)
Use release or self-timer of the camera.
8 Make exposure.
To Next Page
Loading...
Need help?
General
