3 Microscopy
1-1 Phase Contrast Microscopy
E-17
1-1
Phase Contrast Microscopy
In order to perform phase contrast microscopy, the following items are required: an ELWD
condenser, objectives for phase contrast microscopy, a phase slider (either non-centerable or
centerable), an annular diaphragm Ph2 (when Ph2 objective is used), a GIF filter and a centering
telescope (only when a centerable phase slider is used).
Before proceeding to the following operations, perform steps 1 to 6 in “1 Microscopy”.
1
Attach the ELWD condenser.
Attach the ELWD condenser to the illumination pillar.
2
Attach the phase slider.
Insert the phase slider into the condenser.
(When you are planning to use a Ph2 objective, attach the annular diaphragm Ph2 to the slider
in advance.)
3
Insert a GIF filter.
Insert a GIF filter into the filter holder and bring it into the optical path. (Voluntary.)
The GIF filter will improve the contrast of the phase contrast image.
4
Fully open the aperture diaphragm.
Move the aperture diaphragm lever on the condenser to the rightmost to fully open the aperture.
Always fully open the aperture diaphragm for phase contrast microscopy. If the aperture
diaphragm is closed, it will obstruct the annular diaphragm and the phase contrast effects cannot
be obtained.
Annular diaphragm Ph2
Phase slider
ELWD condenser
Aperture
diaphragm
fully open.
Filter holder
GIF filter
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